FastQCFastQC Report
Wed 15 Apr 2020
FCHHWFYBBXX_L3_CHKPEI85217070034_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameFCHHWFYBBXX_L3_CHKPEI85217070034_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100736229
Sequences flagged as poor quality0
Sequence length49
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG8293010.8232400678806431No Hit
CACACACACACACACACACACACACACACACACACACACACACACACAC6581750.6533647393134003No Hit
CACACACACACACACACACACCTGTCTCTTATACACATCTCCGAGCCCA1843000.18295304661444098No Hit
CACACACACACACACACACACACCTGTCTCTTATACACATCTCCGAGCC1800060.17869042923971276No Hit
GTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCTCCGAGCC1667830.16556406930817313No Hit
GTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCTCCGAGCCCA1613360.16015687861414785No Hit
CACACACACACACACACACACACACCTGTCTCTTATACACATCTCCGAG1570390.15589128316486814No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCTCCGAG1511250.1500205055323244No Hit
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC1505730.14947253981484654No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAG1501530.14905560937763512No Hit
CACACACACACACACACACCTGTCTCTTATACACATCTCCGAGCCCACG1356830.13469136312418445No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCTCCG1339050.1329263576066561No Hit
CACACACACACACACACACACACACACCTGTCTCTTATACACATCTCCG1338380.13285984727500572No Hit
GTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCTCCGAGCCCACG1166350.11578257510512927No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCTC1154440.11460027950817972No Hit
CACACACACACACACACACACACACACACCTGTCTCTTATACACATCTC1133960.11256724728101546No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATC1013700.10062913909552838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG480800.024.73930243
AGCCCAC540650.022.12780842
CCCACGA203200.019.66376142
CCACGAG204950.019.49586143
CGAGCCC1016900.017.6035642
GAGCCCA1021250.017.4633743
CCGAGCC1396050.012.99191643
GGGTATA77350.012.9898291
TCCGAGC1440550.012.64128542
CTATACT352550.012.2905334
CCTATAC309050.011.8655763
TATACTG457550.011.639135
GTCCTAC87550.011.4764521
TAACAGT499700.011.4484684
GTATTAA208050.011.3066741
TTATACT190900.011.2983064
GTACTAA147000.011.1620811
GCCTATA298600.011.0389962
GGCCTAT320500.010.9119071
GTATTAT221600.010.907211
Produced by FastQC (version 0.11.5)